Estimating Metabolite Concentrations at Steady State

Biosynthetic Pathway-Funnel Analogy

In a follow up to our post on sequential biochemical pathways, we next wanted to present an method to approximate the concentration of a metabolic intermediate in a biosynthetic pathway. In general, under steady state conditions, the steady state concentration of a metabolite can be estimated from the ratio of the Vmax for the upstream rate-determining enzyme over the rate of decay of that metabolite. A more complete equation is detailed below and further discussed in our post on Estimating Protein/Metabolite Levels from RNAseq data.

Biosynthetic Pathway-Funnel Analogy

 

REFERENCES: 

  1. Segel, I.H. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems, Wiley-Interscience, 1993
  2. Banford, C.H.; Tipper, C.F.H. Comprehensive Chemical Kinetics. Elsevier, 1969
  3. McBane, G.C. Graduate Chemical Kinetics Lecture Notes. OSU875, http://faculty.gvsu.edu/mcbaneg/chem875.pdf
  4. Lauffenburger, D.A. Receptors: Models for Binding, Trafficking and Signalling, Oxford University Press 1993.

 

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This work by Eugene Douglass and Chad Miller is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

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